Functional Characterization and Shelf-Life Evaluation of Purple Tea

client

Purple Tea

Our Role

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Today’s Task

What We did Today?

Event: Functional Characterization and Shelf-Life Evaluation of Purple Tea 

 

Today’s task is focussed on comprehensive functional characterization and shelf-file evaluation of functional purple tea. The study included quantification of phenolic compounds and antioxidant activity using validated analytical methods. Physicochemical parameters such as pH, total soluble solids, colour, and turbidity were monitored alongside microbiological testing to verify product safety throughout storage. Sensory evaluation was conducted to assess changes in appearance, colour, aroma, taste, and overall acceptability at predetermined storage intervals. The collected data were used to determine bioactive retention, monitor quality changes over time, and verify that the beverage maintained acceptable functional, sensory, and microbiological characteristics during the storage period. 

😊 How I Felt Before Starting 

Before starting this experiment, I felt it would be challenging because I had to track several quality changes in the same product over different storage intervals. I was unsure whether the purple tea’s colour and functional compounds would remain stable or change gradually, and whether these changes would also affect its sensory quality. Maintaining consistency while comparing all these changes over time felt like the most challenging part of the study. 

purple tea
purple tea
purple tea
purple tea

🧪 What We Did Today? 

Product Evaluation Plan 

The evaluation was conducted to determine which purple tea beverage variant best retained its characteristic bioactive profile while maintaining acceptable product quality and consumer acceptability throughout storage. The study focused on verifying functional quality, monitoring storage-related changes, and generating scientific data to support product quality validation and commercial shelf-life assessment. 

The evaluation covered: 

  1. Physicochemical characterization  
  2. Functional bioactive characterization  
  3. Sensory profiling  
  4. Storage stability evaluation  

Key Steps Followed During Product Development

Two purple tea beverage variants were prepared using aqueous extracts of purple tea leaves (Camellia sinensis var. TRFK 306) as the primary functional ingredient. The variants differed in the concentration of purple tea extract and the balance of complementary natural ingredients, allowing comparison of functional performancesensory quality, and storage stability while protecting proprietary formulation details. All variants were processed under identical manufacturing conditions to ensure that observed differences were primarily associated with formulation composition rather than processing variations. 

Physicochemical analysis was performed to establish the baseline quality of each purple tea variant and monitor changes that could influence product stability during storage. Parameters including pH (AOAC 981.12)total soluble solids (AOAC 932.12)colour (CIE Lab*), and titratable acidity (AOAC 942.15) were measured to evaluate beverage consistencycolour retentionacid balance, and processing suitability. 

Purple tea is naturally rich in polyphenols, anthocyanins, catechins, and other antioxidant compounds that contribute to its functional value. Therefore, total polyphenols (ISO 14502-1) and antioxidant activity (DPPH assay) were analysed to compare the functional characteristics of the variants and establish reference values for bioactive retention during storage. 

Sensory evaluation was conducted to understand how the phenolic-rich composition of purple tea influenced the beverage’s sensory characteristics and consumer perception. Using ISO 13299 descriptive analysis and a 9-point Hedonic Scale, attributes including colour, aroma, flavour, bitterness, astringency, mouthfeel, and overall acceptability were assessed to identify the variant offering the best balance between functional properties and sensory appeal. 

Storage stability studies were performed under accelerated and real-time conditions to evaluate how each variant performed during storage and whether it could maintain its functional quality throughout the intended shelf life. Periodic monitoring included physicochemical characteristicsbioactive compound retentionantioxidant activitycolour stabilitymicrobiological quality, and sensory attributes. The collected data were used to determine the effect of storage on product quality, verify shelf-life performance, and assess overall product stability. 

📚 What I Learned? 

Observations & Learnings 

  • Purple tea demonstrated a high level of naturally occurring bioactive compounds, including functional constituents, confirming its suitability as a functional beverage.  
  • Bioactive compounds and antioxidant activity were retained at acceptable levels throughout the storage study, indicating good stability under the evaluated conditions.  
  • Monitoring physicochemical parameters such as pH, total soluble solids, titratable acidity, and colour was essential for assessing product consistency and detecting quality changes during storage.  
  • Sensory evaluation showed that colour, aroma, flavour, mouthfeel, bitterness, and astringency remained within acceptable limits over the storage period, indicating acceptable sensory stability during storage. 
  • Periodic microbiological assessment confirmed that the beverage remained within the specified quality limits throughout storage.  
  • Integrating functional, physicochemical, sensory, and microbiological evaluations

Outcome (Analysis Table) 

Interpretation 

Both purple tea variants maintained good physicochemical stability, functional quality, and sensory acceptability over 90 days. The Mixed Berry variant showed better bioactive retention, with total polyphenols decreasing from 878 to 831 mg GAE/L compared with 845 to 796 mg GAE/L for Citrus. Anthocyanins decreased from 55.3 to 48.6 mg/L (Mixed Berry) and 48.6 to 42.5 mg/L (Citrus), while DPPH antioxidant activity remained higher in Mixed Berry (90.2% to 86.5%) than Citrus (88.5% to 84.8%). Mixed Berry also maintained slightly higher overall acceptability (8.7 to 8.3) than Citrus (8.5 to 8.1), indicating superior storage stability among the evaluated variants.

✅ What Went Well? 

  • Preserved high levels of polyphenols, anthocyanins, and antioxidant activity throughout the 90-day storage study.  
  • Maintained stable physicochemical properties, including pH, colour, and total soluble solids, under the evaluated storage conditions.  
  • Achieved good sensory acceptability by retaining the characteristic colour, flavour, and balanced astringency of purple tea during storage.  
  • Demonstrated good microbiological stability and overall product quality throughout the storage period.  
  • Generated comprehensive data supporting functional characterization and shelf-life validation. 

⚠️ What Could I Have Done Better? 

  • Extend real-time shelf-life studies to 6–12 months to confirm long-term retention of polyphenols, anthocyanins, and antioxidant activity.  
  • Evaluate light- and oxygen-barrier packaging to better protect the naturally occurring anthocyanins responsible for the purple colour.  
  • Perform LC-MS/HPLC profiling to identify and quantify individual anthocyanins, catechins, and other polyphenolic compounds during storage.  
  • Include additional antioxidant assays such as ORAC alongside DPPH, ABTS, and FRAP for a more comprehensive assessment of antioxidant capacity.  
  • Investigate the influence of different storage temperatures and light exposure on colour stability, bioactive retention, and sensory quality. 

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